State 5 objectives of protein fractionation?

2.Explain the major differences between stacking and resolving gel?

3.Explain the principle of flourescent spectroscopy?

4.Describe "salting out" technique in purification of macromolecules?

5.Outline the operational principle of isoelectric focusing point?

6.State the advantage s of Enzyme linked immunosorbent assay?

7.Explain why polyacrylamide gel cannot be used to separate DNA?

8.Describe the principle of sedimentation technique of separation of subcellular organelles.