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Study the interactions between 3 genes A, B, and C, you have generated a homozygous triple mutant line. Mutation A is associated with a
Study the interactions between 3 genes A, B, and C, you have generated a homozygous triple mutant line. Mutation A is associated with a dominant orange eye color phenotype, mutation B with a dominant yellow eye color phenotype and both Mut A and Mut B affect genes on the second chromosome, while mutation C has no visible phenotype and is located on the third chromosome. (Note that the eye color phenotype associated with mutations A and B is additive). One day you want to send to a collaborator the 3 original single mutant strains, but you discover that a graduate student in the lab have discarded them all. How can you regenerate the 3 missing stocks starting from the triple mutant you have and the following 2 stocks: W/w (or Y); If/SMS; TM3/TM6 and w/w (or Y); +/ +:+/+. Write the different steps/crosses that you will perform to reach your aim, showing the complete genotypes at each step and briefly explain your strategy and how do you select the progeny of interest after each cross (based on which phenotypes).
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