Suppose a protein exists in two forms: R and T. These forms are in equilibrium RTRT .for which K=[T][R]=10.0K=[T][R]=10.0 . R and T are in turn in equilbrium with their ligand-bound forms according to R+LRLR+LRL for which kR=[RL][R][L]=2.00kR=[RL][R][L]=2.00 and T+LTLT+LTL for which kT=[TL][T][L]=0.100kT=[TL][T][L]=0.100

Binding polynomial q sub b / R is 15.5 when L is 1.5

Suppose a protein exists in two forms: R and T. These forms are in equilibrium RT for which T K= = 10.0. R and T are in turn in equilbrium with their ligand-bound forms according to R+LRL for [R] (TL) which kg = [RL] [RL = 2.00 and T+L=TL for which kr = = 0.100 TIL Part B R For the conditions described above, calculate the fraction of R sites bound fB VO 6 t o ? SB - = Part C For the conditions described above calculate the fraction of T sites bound f VO ADD 1 t o ? f La Gm 11 Submit Previous Answers Request Answer Part D If [R]=0.010, using the conditions given above calculate the concentration of TL i.e. [TL]. VE a GRAN B ? (TL) T 1