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.. Suppose that instead of measuring fluorescence intensity at an emission wavelength of 513 nm, the fluorescence intensity of all of the standard addition solutions

.. Suppose that instead of measuring fluorescence intensity at an emission wavelength of 513 nm, the fluorescence intensity of all of the standard addition solutions was measured at an emission wavelength of 575 nm (see your qualitative pre scan spectrum). Fully explain how this would affect the concentration of fluorescein (mg/jug) determined in the unknown antifreeze sample.

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