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Surface phage display is a commonly used method for testing method that allows for the presentation of a protein of interest on the surface

 

Surface phage display is a commonly used method for testing method that allows for the presentation of a protein of interest on the surface of the bacteriophage. How would one genetically create proteins on the surface of bacteriophages? In other words, what is required to make your genetic construct for phage display? Finally, why would you want to repeat the affinity selection process a couple times?

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