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Scientists can track the movement of proteins through the endomembrane system using an approach known as a pulse-chase experiment. This experiment involves the pulse phase

Scientists can track the movement of proteins through the endomembrane system using an approach known as a pulse-chase experiment. This experiment involves

the “pulse” phase: Cells are exposed to a high concentration of a radioactively labeled amino acids for a short period to “tag” proteins that are being synthesized.

the “chase” phase: Any unincorporated (not used to make proteins) radioactively labeled amino acids are washed away and large amounts of the same, but unlabeled, amino acid are added.

Only those proteins synthesized during the brief pulse phase are radioactively tagged. These tagged proteins can be tracked through the chase period to determine their location in the cell.

The data below were obtained from a pulse-chase experiment in which cells were examined at different times during the chase period. The numbers represent the radioactivity (measured in counts per minute) recorded at each of the indicated sites. The higher the number, the greater the radioactivity.

Table 1. Results of pulse-chase experiment.

 

Radioactivity Levels (in counts/minute) by cellular location

Time After Pulse

ER

Golgi

Cytoplasm

Lysosomes

Extracellular Space

3 min

162

7

21

5

5

20 min

73

88

17

8

10

60 min

9

35

14

11

112

120 min

11

23

18

12

151



1. Examine the narrative and table provided above and provide a 1 sentence explanation (in your own words) of the information the table conveys.

2. Based on the data and your interpretation, predict what is the most likely function of the cells utilized in this experiment? Explain your answer.

 

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Required solution Ans 1 The table gives the result of pulse chase experiment It is a two phase technique used to examine cellular processes that take place over a period of timeDuring the pulse phase ... blur-text-image

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