Task 1) - Glucose calibration curve:

a) plot the measured absorbances (y-axis) against the glucose concentrations (indicated in mg/ml, mg glucose/reaction preparation and glucose concentration in mM) (x-axis).

b) plot the measured absorbances (y-axis) against time (x-axis), use one and the same diagram for all four curves!

c) From the plateau of the curves, determine the values with the highest absorbance (end point) and use the glucose calibration curve to determine the amount of glucose formed during hydrolysis!

d) Use the value of batch 8 of the acid hydrolysis as the value of the maximum conversion (100 % = complete cleavage of glycogen into glucose) and calculate the percentage value of the maximum conversion for the enzymatic hydrolysis! maximum conversion!

e) What is the maximum amount of glucose that could have been released? Compare this value with the amount of glucose released during acidic hydrolysis!

concentrations of glucose Measured values for the calibration curve (A530nm) (from the first chart above) readings from acid hydrolysis of starch and dextran: (A530nm) (from the second chart above) Measured values enzymatic glucose determination (A530nm) (readings) (from the third chart above) concentrations of glucose Measured values for the calibration curve (A530nm) (from the first chart above) readings from acid hydrolysis of starch and dextran: (A530nm) (from the second chart above) Measured values enzymatic glucose determination (A530nm) (readings) (from the third chart above)