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The diagram below shows how the activities of 3 different enzymes are affected by pH at constant temperature. 100+ A B im Enzyme activity (%)

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The diagram below shows how the activities of 3 different enzymes are affected by pH at constant temperature. 100+ A B im Enzyme activity (%) 0- pH Which enzyme activity is unlikely to be associated with mammalian cells? Select one: O a. Enzyme A O b. Enzyme B O c. Enzyme C Select the true statement from below about the main forms of aspartic acid present at its isoelectric point Select one: O a. all have a positive overall charge O b. only the main form has a neutral overall charge O c. They are all neutral in charge O d. They include a mixture of positive, negative and neutral forms e. They are all either positively or negatively charged Garry performs an assay for protease function using casein as substrate. He finds it difficult to interpret the resulting plot (Figure left), so he alters the experimental protocol and collects another data set (Figure right), 100 100 75 75 50 50 . . 25 . 4 pH pH Which one of the following changes did Garry most likely make to his protocol? Select one: O a. Incubate the reactions at a lower temperature O b. Use more enzyme in each reaction O c. Incubate the reactions for a shorter time period

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