The following data are for a liquid chromatographic column

Length of packing 24.7 cm

Flow rate 0.313 ml/min

VM 1.37 ml

VS 0.164 mL

A chromatogram of a mixture of species A, B, C, and D provided the following data:

	Retention Time, min	Width of peak Base (W), min
Non-retained	3.1	-
A	5.4	0.41
B	13.3	1.07
C	14.1	1.16
D	21.6	1.72

Calculate

(a) the number of plates from each peak.

(b) the mean and the standard deviation for N.

(c) the plate height for the column.

From the data in Problem ,calculate for A, B, C, and D

(a) the retention factor.

(b) the distribution constant

From the data in Problem, calculate for species B and C

(a) the resolution.

(b) the selectivity factor (alpha).

(c) the length of column necessary to separate the two species with a resolution of 1.5.

(d) the time required to separate the two species on the column in part (c).

From the data in Problem , calculate for species C and D

(a) the resolution.

(b) the length of column necessary to separate the two species with a resolution of 1.5.