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The following data was obtained after processing a chromatogram of an antibody and its main impurity table [ [ Impurity , Antibody ] ,

The following data was obtained after processing a chromatogram of an antibody
and its main impurity \table[[Impurity,Antibody],[Time(min),C(ug/ml),Time(min),C(ug/ml)],[,,,],[,,,],[4.090,0.000,4.310,0.008],[4.538,0.000,4.745,0.029],[4.793,0.000,4.917,0.053],[5.221,0.000,5.152,0.127],[5.428,0.000,5.228,0.167],[5.828,0.000,5.386,0.273],[6.021,0.002,5.490,0.329],[6.393,0.012,5.655,0.457],[6.545,0.024,5.717,0.512],[6.848,0.059,5.890,0.651],[6.986,0.084,5.979,0.708],[7.228,0.155,6.179,0.798],[7.538,0.261,6.572,0.729],[7.641,0.290,6.683,0.667],[7.924,0.371,6.931,0.482],[8.366,0.398,7.207,0.269],[8.503,0.378,7.303,0.212],[8.710,0.314,7.593,0.086],[8.814,0.280,7.745,0.047],[9.028,0.206,8.269,0.006],[9.138,0.175,8.600,0.000],[9.372,0.110,9.317,0.000],[9.510,0.075,9.628,0.000],[9.883,0.024,10.193,0.000],[10.103,0.010,,],[10.566,0.004,,],[10.800,0.004,,]]
A) Reconstruct the original chromatogram.
B) Considering that the number of theoretical plates is 150 and the flowrate is 0.5 mL/min. Plot the
yield and purity as function of time if fractions are collected after 4.4 minutes.
C) At what time you should stop collecting antibody if it is desired a purity >98%
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