The following is an image of Coommassie Blue dye stained SDS-PAGE gel. The details of gel lanes are given below:

a) Lane 1 and 7 are low molecular weight protein standards (Phosphorylase b- 97,400, BSA-66,200, Ovalbumin-45,000, Carbonic anhydrase-31,000, Trypsin inhibitor-21,500, and Lysozyme-14,400) and lane 9 is a prestained protein standards.

b) Lane 3 and 5 are ammonium sulfate fractionated partially purified proteins from eggwhite.c) Use Lane 7 and Lane 5 to measure protein migration (use ruler shown on right hand side) and answer

questions below:

i) How many bands are in the ammonium sulfate partially purified protein? What does this tell you?

ii) You believe that band shown by an arrow is ?Ovalbumin?. Using low MW standards migration patterns determine MW of this protein. To better explain prepare a Table (as you did in SDS-PAGE lab) and plot and method how did you obtain the molecular weight?

iii) Is this molecular weight necessarily the molecular weight of the functional protein? Justify your answer with proper explanation.

6 8 6 LO 4 3 - |qu|q 0 cm 1 2 3 A mju u p 4 ||||||| // 5 6