The following is the -adrenergic receptor and a small G protein (shown in blue) oriented in the membrane. Answer parts (a)-(d) by using the options:

The following is the β-adrenergic receptor and a small G protein (shown in blue) oriented in the membrane. Answer parts (a)-(d) by using the options: Cytoplasm/ nucleus/ rough endoplasmic reticulum (RER)/ Smooth endoplasmic reticulum (SER)/ vesicles/ cell membrane. a) Where in the cell does the translation of the following
proteins begin?
• β-adrenergic receptor?
• G protein?
b) Where in the cell is the translation of the following proteins completed?
• β-adrenergic receptor:
• G protein:
c) Where in the cell is the newly translated G protein post-translationally modified by getting covalently coupled to the lipid (shown in red)?
d) Which intracellular structure is involved in transporting these two newly translated proteins to the cell membrane?
e) Which of the two proteins have a signal sequence and what is its significance?
f) On the diagram of β-adrenergic receptor on the previous page, circle the part of β-adrenergic receptor that is not present in secreted proteins.
g) Based on the information in the schematic above, can you label the -NH2 and –COOH ends of the……

 i. β-adrenergic receptor as it is being translated? If yes, draw this as it is being translated. If no, please write “No”.
 ii. G protein? If yes, draw this as it is being translated. If no, please write “No”.

B-adrenergic receptor OUTSIDE CELL (Free amino end) NH, Note: Lipid attached to G protein shown in RED www. wwwww www. G protein JU wwwww www. www. Cytoplasm COOH