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The following results were obtained for the action of an ATPase on ATP at 20C, when the concentration of the ATPase was 20 nmol
The following results were obtained for the action of an ATPase on ATP at 20C, when the concentration of the ATPase was 20 nmol dm -3 [ATP]/(umol dm-3) 0.60 2.0 0.80 1.4 3.0 v/(umol dm3 s) 0.81 0.97 1.30 1.47 1.69 (a) Determine the Michaelis constant. 1.11 umol dm 3 (b)Determine the maximum velocity of the reaction. 2.31 pmol dm 3 s1 (c) Determine the turnover number. X s1 (d) Calculate the catalytic efficiency of the enzyme. dm3 umol s1
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Separation process principles
Authors: J. D. Seader
2nd Edition
471464805, 978-0471464808
