Question
The forward and reverse primers for PCR amplification have thefollowing sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse:5'- GAAAGCATCCAGGCCCGG-3'. Based on the manually calculated meltingtemperatures, would you recommend using
The forward and reverse primers for PCR amplification have thefollowing sequences: Forward: 5'-CTATGAGTTACTCACATG-3', Reverse:5'- GAAAGCATCCAGGCCCGG-3'. Based on the manually calculated meltingtemperatures, would you recommend using these primers together? Whyor why not?
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Genetics Analysis And Principles
Authors: Robert Brooker
5th Edition
978-0073525341, 0073525340
