Question: The gene encoding the novel 17 kDa protein, FURM, was cloned and the predicted primary sequence was used to create the hydropathy plot shown below:

The gene encoding the novel 17 kDa protein, FURM, was cloned and the predicted primary sequence was used to create the hydropathy plot shown below: a)An attempt to determine optimal lysis conditions for extracting FURM from cells was performed by treating the cells with different conditions (see below) and then centrifuging to remove insoluble debris. The resulting supernatants were analysed by Western analysis using a specific antibody raised against FURM. b)Lane 1 - whole cells Lane 2 - soluble fraction after hypotonic lysis Lane 3 - soluble fraction after treatment with 10 mM CAPS buffer (pH 11) Lane 4 - soluble fraction after treatment with 1% sodium dodecyl sulphate Referring to both pieces of data above, what can you conclude about FURM

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