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The inactivation of an enzyme in a product treated with HHP generated the information shown in the table by plotting log (A/Ao) versus time in

The inactivation of an enzyme in a product treated with HHP generated the information shown in the table by plotting log (A/Ao) versus time in minutes at different temperatures and pressures. Using the information shown determine:

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a) k, D, Va, Ea, ZP, ZT values b) Residual enzyme activity curve (A/Ao vs. time) at 400 MPa/30C to inactivate 90% of the enzyme.

400MPa \begin{tabular}{|l|r|} \hline \multicolumn{1}{|c|}{T () } & \multicolumn{1}{c|}{ slope } \\ \hline 25 & -0.02 \\ \hline 35 & -0.059 \\ \hline 45 & -0.092 \\ \hline \end{tabular} 600MPa \begin{tabular}{|l|r|} \hline \multicolumn{1}{|c|}{T(C)} & \multicolumn{1}{c|}{ slope } \\ \hline 25 & -0.039 \\ \hline 35 & -0.089 \\ \hline 45 & -0.132 \\ \hline \end{tabular}

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