The intrinsic maximum reaction rate of enzyme immobilized on flat sheet is $2{10}^{-6}mo\frac{l}{s}.mg$ enzyme. The amount of enzyme bound on the surface was found to be $5{10}^{-4}mg$ enzyme $\frac{?}{c{m}^2}$ of immobilized support.

Assume:

$K_L=2{10}^{-5}c\frac{m}{s}$

$S_b=10mM$

$K_m=5mM$

Is the immobilized system mass transfer limited or not. Calculate the immobilization efficiency