The protein to be purified has the following characteristics: Tm = 32C, Hm = 50kcal/mole, Cp= 0.72 kcal/mole.

The dissociation constant between the antibody and the folded protein is 10-8M.

The total protein concentration loaded on the column is 10-7M, and the effective antibody concentration in the packed column is 10-6M.

What fraction of the total protein on the column would be recovered in the final elution step, if carried out at room temperature (21C)?