Question
The protein to be purified has the following characteristics: Tm = 32C, Hm = 50kcal/mole, Cp= 0.72 kcal/mole. The dissociation constant between the antibody and
The protein to be purified has the following characteristics: Tm = 32C, Hm = 50kcal/mole, Cp= 0.72 kcal/mole.
The dissociation constant between the antibody and the folded protein is 10-8M.
The total protein concentration loaded on the column is 10-7M, and the effective antibody concentration in the packed column is 10-6M.
What fraction of the total protein on the column would be recovered in the final elution step, if carried out at room temperature (21C)?
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Auditing and Assurance Services
Authors: Timothy Louwers, Robert Ramsay, David Sinason, Jerry Straws
6th edition
978-1259197109, 77632281, 77862341, 1259197107, 9780077632281, 978-0077862343
