The results obtained when applying high pressures to orange juice are presented below. The results reflect the linear relationship between the decimal logarithm of the value of D (in minutes) versus pressure in MPa for different microorganisms. Generate the microbial population reduction curve for the native flora at 300 MPa considering that the initial microorganism load is 1x106 microorganisms /ml (CFU/ml) and the maximum process time is 15 minutes. What is the activation volume for the inhibition of each microorganism?

Z values for natural flora, S. cerevisiae, E. coli, and L. innocua inactivation in pressurized orange juice