The system investigated in problem 7.2 was investigated again under identical conditions but in the presence of oxaloacetate initial concentration 2.0 mmol 1"1 in each case). These results were obtained: Absorbance (at 340 nm) at time t (minutes) [NAD+] (mmol 1"1) t=0.5 1.0 2.0 2.5 3.0 1.5 1.5 0.026 0.042 0.058 0.074 0.088 0.102 2.0 0.028 0.045 0.062 0.080 0.096 0.112 2.5 0.029 0.047 0.066 0.084 0.102 0.117 3.33 0.030 0.050 0.070 0.090 0.108 0,123 5.0 0.032 0.053 0.074 0.097 0.115 0.130 10.0 0.033 0.057 0.080 0.103 0.124 0.140 (0) Using the absorbance vs time values provided. Calculate initial velocity at each substrate concentration (6 points). [ Do not show calculations, I will mark the values on the graph provided in (ii)]. (ii) Provide a lineweaver burk plot and answer the following questions (a) what type of inhibition is exhibited (b) What is km and Vmax for the inhibited reaction (14 points). NB use a graph paper! Absorbance (at 340 nm) at time t (minutes) [NAD+) (mmol 1"1) t = 0.5 1.0 1.5 2.0 2.5 3.0 1.5 0.026 0.042 0.058 0.074 0.088 0.102 2.0 0.028 0.045 0.062 0.080 0.096 0.112 2.5 0.029 0.047 0.066 0.084 0.102 0.117 3.33 0.030 0.050 0.070 0.090 0.108 0,123 5.0 0.032 0.053 0.074 0.097 0.115 0.130 10.0 0.033 0.057 0.080 0.103 0.124 0.140 Using the absorbance vs time values provided. Calculate initial velocity at each substrate concentration (6 points). [ Do not show calculations, I will mark the values on the graph provided in (ii)]. (ii) Provide a lineweaver burk plot and answer the following questions (a) what type of inhibition is exhibited (b) What is Km and Vmax for the inhibited reaction (14 points). NB use a graph paper