The volume of the chemostat equipped with a reflux system is $1000mL,$ and the

glucose solution flows in at a rate of $F=1m\frac{L}{m}in.$ The cell concentration ratio

of the recycle stream is $2\mathrm{.}5,$ the recycle flow rate ratio is $0\mathrm{.}25,$ and it is operated

in a steady state. Answer the questions using the constants below.

$Y\frac{x}{S}=0\mathrm{.}5g$ cells $\frac{?}{g}$ glucose

$So=50g$ glucose $\frac{?}{L}$

umax $=0\mathrm{.}2h^{-1}$

$Ks=1g$ glucose $\frac{?}{L}$

Find the substrate concentration, $S,$ of the reflux flow $($unit: $\frac{g}{L}).$

Find the specific growth rate $($unet$)$ of the cell $($unit: reciprocal of $h).$

Find the cell concentration in the reflux flow $($unit: $\frac{g}{L})$

Find the cell concentration of the flow discharged from the cell separator $($unit:

$\frac{g}{L}$