There are two drugs P and Q that inhibit AK2's activity. You only have 3 nmoles of AK2 remaining from your purification. You decide to test these inhibitors in the lab. You generate the following dataset in three separate 10mL reactions that you follow spectrophotometrically. Vo Vo Vo (mM/sec) (mM/sec) (mM/sec) [Substrate] (mM) Control + Inh. P + Inh. Q 10 22.30 6.98 17.53 20 37.07 12.88 24.97 40 58.63 24.35 36.30 60 74.60 33.73 40.33 80 80.58 39.22 41.99 100 83.55 46.24 46.63 125 85.34 50.94 45.02 150 88.40 55.87 45.06 180 87.11 59.64 47.34 Calculate: 4) Kinetic parameters Vmax, Km and turnover number for AK2. 5) The apparent Km when the inhibitors are added. 6) The type of inhibition caused by each of the compounds. Justify why you think this may be the case.Vo (mM/sec) Vo (mM/sec Vo (mM/sec) [Substrate] (mM) Control Inh. P Inh. Q 1/[S] 1/Vo (Control) 1/Vo (Inh. P) 1/Vo (Inh. Q) 10 22.3 6.98 17.53 0.10000000 0.04484305 0.14326648 0.05704507 20 37.07 12.88 24.97 0.05000000 0.02697599 0.07763975 0.04004806 40 58.63 24.35 36.3 0.02500000 0.01705611 0.04106776 0.02754821 60 74.6 33.73 40.33 0.01666667 0.01340483 0.02964720 0.02479544 80 80.58 39.22 41.99 0.01250000 0.01241003 0.02549720 0.02381519 100 83.55 46.24 46.63 0.01000000 0.01196888 0.02162630 0.02144542 125 85.34 50.94 45.02 0.00800000 0.01171783 0.01963094 0.02221235 150 88.4 55.87 45.06 0.00666667 0.01131222 0.01789869 0.02219263 180 87.11 59.64 47.34 0.00555556 0.01147974 0.01676727 0.02112379