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To quantify the amount of beta-galactosidase induction, an enzyme assay for product formation was allowed to proceed for exactly 5 min before stopping the reaction

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To quantify the amount of beta-galactosidase induction, an enzyme assay for product formation was allowed to proceed for exactly 5 min before stopping the reaction with Na cos. The assay solution (1.0 ml) was centrifuged and 300 ul of the supernatant was pipetted into a microtitre plate. The Acos was read and the value (after correction for non-enzymic hydrolysis) was found to be 0.8. Assume the length of the light path was 0.3 cm, and the scos of the product is 21300 M-cm The approximate concentration of the product was: The approximate number of umoles of product in the mi assay solution was: Given that one unit of beta-galactosidase activity is the conversion of one umoto of substrate to product in one minuto, calculate the number of enzyme units in the volume assayed

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