triacylglycerol, cholesterol, phosphatidylcholine, and sphingomyelin. 2. Identify the polar/charged groups and draw how water will orient itself around these groups. 3. Order these lipids from most hydrophobic to most hydrophilic. 4 Predict how the lipids will migrate on the TLC plate when the running buffer is an organic solvent. Keep in mind that although the lipid standards are dissolved in organic solvent, they can (by virtue of their polar/charged groups) interact with the polar silica matrix of the TLC plates