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Two groups of students conducted an experiment. They were supposed to use 10 mg/mL NAD and 10 mg/mL NADH and record absorbances_(figure 1.) group 2,
Two groups of students conducted an experiment. They were supposed to use 10 mg/mL NAD and 10 mg/mL NADH and record absorbances_(figure 1.) group 2, however, made a mistake and used 0.1 MM NAD' and 0.1 mM NADH instead, and recorded their absorbances (figure 2). Molecular mass of acid NAD and NADH is 663.43 g/mol and 709.40 g/mol, Figure 1 Figure 2 0.9 0.9 0.8 0.8 0.7 0.6 O.S 0.7 0.6 0.5 beroence (510 m) Absorbance (510nm 04 0.4 0.3 0.2 0.3 - 0.2 0.1 0.1 0.0 0.0 NADH NAD None NADH NAD+ None i) ii) Describe the trends What has happened. Why didn't group 2's work
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