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Using the electrospray ionization spectrum shown below. 1) Please determine the molecular weight of this protein and assign the correct change state to each peak.

Using the electrospray ionization spectrum shown below. 1) Please determine the molecular weight of this protein and assign the correct change state to each peak. 2) Surface activity is a physical property of a molecule. Which model of electrospray would you expect to see an increase in response for surface-active species (coulombic explosion or ion evaporation)? Why?

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100 849 893 808 943 771 Relative abundance 998 1061 738 707 779 745 1131 1212 (1088 1151 1226 Tel. 1050 1100 1150 1200 0 700 750 800 850 900 950 1000 100 849 893 808 943 771 Relative abundance 998 1061 738 707 779 745 1131 1212 (1088 1151 1226 Tel. 1050 1100 1150 1200 0 700 750 800 850 900 950 1000

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