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Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell-specific growth rate and doubling time, (b) cell-specific glucose consumption and lactate production rates,
Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell-specific growth rate and doubling time, (b) cell-specific glucose consumption and lactate production rates, and (c) the cell-specific IgG production rates. Calculate these parameters using differential and integral methods. For (a) and (b) focus only on the exponential phase of the growth while (c) perform the analysis for the whole culture period.
Days in Culture | Viable cell density | Viability | Nova pH | Nova Glucose (Gluc) g/L | Nova Lactate (Lac) g/L | Titer (mg/L) | |
0.0 | 1.87E+05 | 90.30 | 7.201 | 6.1 | 0 | 0 | |
1.4 | 3.86E+05 | 95.20 | 7.042 | 6 | 0.05 | 18.05 | |
2.3 | 6.40E+05 | 93.90 | 7.074 | 5.8 | 0.1 | 29.55 | |
2.7 | 8.00E+05 | 93.80 | 7.064 | 5.5 | 0.3 | 36.65 | |
3.3 | 1.15E+06 | 93.60 | 6.916 | 5.2 | 0.5 | 58.5 | |
3.9 | 1.73E+06 | 93.60 | 6.639 | 4.75 | 0.8 | 65 | |
4.5 | 2.40E+06 | 88.80 | 6.688 | 4.1 | 1.1 | 70.5 | |
5.0 | 3.76E+06 | 92.40 | 6.698 | 3.1 | 1.8 | 135.5 | |
5.7 | 4.94E+06 | 93.20 | 6.683 | 2 | 2.8 | 189.5 | |
6.2 | 6.70E+06 | 92.10 | 6.666 | 0.5 | 3.89 | 250.5 | |
6.9 | 8.09E+06 | 91.60 | 6.692 | 0 | 4.1 | 368.5 | |
7.4 | 8.86E+06 | 88.70 | 6.743 | 0 | 4.05 | 495 | |
8.1 | 1.02E+07 | 87.70 | 6.73 | 0 | 4 | 641.5 | |
8.6 | 1.17E+07 | 87.00 | 6.75 | 0 | 3.8 | 767.6 | |
9.5 | 1.23E+07 | 84.60 | 6.885 | 0 | 3.76 | 962.5 | |
9.9 | 1.24E+07 | 81.80 | 6.81 | 0 | 3.7 | 1148 | |
10.6 | 1.35E+07 | 79.10 | 6.851 | 0 | 3.44 | 1366 | |
11.1 | 1.31E+07 | 76.10 | 6.851 | 0 | 3.4 | 1575 | |
11.8 | 1.27E+07 | 72.70 | 6.964 | 0 | 3.2 | 1756 | |
12.3 | 1.17E+07 | 63.70 | 6.919 | 0 | 3.4 | 1962 | |
12.9 | 9.84E+06 | 59.20 | 6.961 | 0 | 3.2 | 2112 | |
13.4 | 8.87E+06 | 47.90 | 6.985 | 0 | 3.12 | 2215 | |
14.1 | 7.15E+06 | 40.80 | 7.044 | 0 | 3 | 2328 | |
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ANSWER a Cellspecific growth rate and doubling time Differential method The cellspecific growth rate can be calculated using the following equation lnN2N1t2t1 where N1 is the viable cell density at ti...
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