Question
We wish to study the effect of an uncompetitive inhibitor on an isomerase. the tests are carried out without adding the inhibitor, yielding the following
We wish to study the effect of an uncompetitive inhibitor on an isomerase. the tests are carried out without adding the inhibitor, yielding the following results:
subsequently the inhibitor is added, in such a way that the [ES] / [ESI] ratio is 0.25
Calculate:
a. Km, Vmax, Apparent Vmax
b. Predict how much the initial velocity decreases once the inhibitor is added, with a [S]= 3.5
[S] (MM) 0,5 3,5 Vo (mM/s) 23,5 44
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a we calculate Kun And Vwux Apparent Vmax 05 M 235 Ms and ...
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Biochemistry Concepts and Connections
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
1st edition
321839927, 978-0133853490, 133853497, 978-0321839923
