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What are the main methods to introduce non canonical AAs in proteins? No partial credit! using a tRNA/aatRNA synthetase pair orthogonal to the organism used

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What are the main methods to introduce non canonical AAs in proteins? No partial credit! using a tRNA/aatRNA synthetase pair orthogonal to the organism used Functionalizing CA/A with the desired amino acid, and ligating to a tRNA enzymatically feeding a non canonical amino acid to the cell and hope for the best Charging a tRNA synthetically with the desired amino acid

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