What is the approximate molecular weight of the unknown protein? Prepare a graph 12. You have isolated an unknown protein and wish to estimate its molecular weight using column gel filtration with several standard proteins. The gel material is Sephacryl S-100. The following elution volumes were measured Protein Hemoglobin Egg albumin Chymotrypsinogen Myoglobin Cytochrome Unknown MW 64,500 45,000 23,200 16,900 13,000 2 Elution volume (mL) 10.1 145 250 28.2 310 7.5 Screen dipping taken: 2/28/2017 9 31 PM