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What is the effect of each of the following treatments on the oxygen affinity of hemoglobin A in vitro and why? 1. Increase in pH

What is the effect of each of the following treatments on the oxygen affinity of hemoglobin A in vitro and why?

1. Increase in pH from 7.2 to 7.4
2. Increase in pCO2 from 10 to 40 torr

3. Increase in [2,3-BPG] from 2 x 10-4 to 8 x 10-4M

4. Dissociation of a2ß2 into monomeric subunits

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