Question
The figure below shows the fluorescence emission spectra of fluorescein (FI) and Texas-Red (TR) when covalently attached to the catalytic (C) or regulatory (R) subunit
The figure below shows the fluorescence emission spectra of fluorescein (FI) and Texas-Red (TR) when covalently attached to the catalytic (C) or regulatory (R) subunit of d cAMP-dependent protein kinase (PKI). When the subunits are associated, resonance energy transfer (RET) occurs from FL to TR. The associated from is C2R2. Read the figure legend and provide a possible explanation for the spectral data.
80 60 40 20 520 580 640 700 WAVELENGTH (nm) FLUORESCENCE
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Consider the first peak is raised with respect to fluorescein and second peak with res...
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Organic Chemistry
Authors: Graham Solomons, Craig Fryhle, Scott Snyder
11th edition
1118133579, 978-1118133576
