Question
When you purchase T 4 DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl 2 , 100mM dithiothritol, 70mM ATP, 450ug/ml bovine serum
When you purchase T4 DNA Ligase, a 10X buffer (400mM Tris, pH 7.5; 200mM MgCl2, 100mM dithiothritol, 70mM ATP, 450ug/ml bovine serum albumin) is supplied with it. What are the final concentrations of MgCl2 and bovine serum albumin when you use the ligase enzyme to perform a ligation reaction?
SHOW WORK
Step by Step Solution
3.57 Rating (154 Votes )
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started
Auditing and Assurance Services Understanding the Integrated Audit
Authors: Karen L. Hooks
1st edition
471726346, 978-0471726340
