Which of the following emission filter sets would be most appropriate for imaging the results using fluorescence microscopy? Note: Light of the given wavelength parameters shown below may pass through the indicated filter set (ie, < 650 nm means that light with A < 650 nm can pass). Fluorescence emission (A) Alex Fluor 488, < 650 nm; Alex Fluor 647 < 750 nm (B) Alex Fluor 488, < 550 nm; Alex Fluor 647 > 600 nm (C) Alex Fluor 488, > 500 nm; Alex Fluor 647 > 600 nm (D) Alex Fluor 488, > 450 nm; Alex Fluor 647 < 750 nm (E) None of the above 400 12 450 34 567 89 10 1112 13 14 15 16 17 18 500 600 650 Wavelength (nm) 550 700 750 19 800 850 I 1. Alexa Fluor 405 2. Alexa Fluor 350 3. Alexa Fluor 500 4. Alexa Fluor 488 5. Alexa Fluor 430 6. Alexa Fluor 514 7. Alexa Fluor 532 8. Alexa Fluor 555 9. Alexa Fluor 546 10. Alexa Fluor 568 11. Alexa Fluor 594 12. Alexa Fluor 610 13. Alexa Fluor 633 14. Alexa Fluor 635 15. Alexa Fluor 647 16. Alexa Fluor 660 17. Alexa Fluor 680 18. Alexa Fluor 700 19. Alexa Fluor 750 1