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Which of the following is true for Michaelis-Menten enzymekinetics? The lower the K m , the higher affinity of thesubstrate to the enzyme The value

Which of the following is true for Michaelis-Menten enzymekinetics?

  1. The lower the Km, the higher affinity of thesubstrate to the enzyme
  2. The value for Km is independent of temperature
  3. Vmax is unimportant because we measure1/Vmax to find Km
  4. Km is calculated after the reaction reachesequilibrium

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