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Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from an ANION EXCHANGE column with diethylamino groups
Which of the following methods would be expected to help elute (i.e., WEAKEN the binding of) proteins from an ANION EXCHANGE column with diethylamino groups in the stationary phase and a mobile phase with pH=8.0. Each correctly assigned choice (i.e., either checked or not) earns 0.25 marks. Each incorrectly assigned choice earns 0 marks. Increasing the concentration of salt (NaCl) in the mobile phase from 0.1M to 0.5 M for a protein with pl=5.0. Increasing the pH of the mobile phase from 8.0 to 9.0 for a protein with pl=7.0. Decreasing the pH of the mobile phase from 8.0 to 7.0 for a protein with pl= 7.5. Decreasing the pH of the mobile phase from 8.0 to 7.0 AND increasing the concentration of salt (NaCl) from 0.1M to 0.5M for a protein with pl=6.0
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