will like for complete answer for question #2 - please provide graphs and vmax and km

Question 1 data: \begin{tabular}{r|r|} {[S]mM} & V0(mM/s) \\ \hline 0.2 & 1.75381 \\ \hline 0.5 & 3.24371 \\ 1 & 4.52511 \\ 2 & 5.63890 \\ \hline 5 & 6.61596 \\ \hline 10 & 7.02150 \\ \hline \end{tabular} Assignment 2 Directions: Please follow these directions and write your final answers on the Answer Sheet on Page 1. 1. Let's get some practice determining Vma and Ku for a generic encyme. The data table for initial rates for the reaction for various substrate concentrations is on Canvas and will look like the table below. Please open it. a. First, using Excel (preferred) or by graphing by hand, graph the initial rate V0 vs. [S] ( V0 goes on the y-axis and [S] goes on the x-axis). This is a Michaelis-Menton plot. Label the axes with proper units and title it "Michaelis-Menton Plot" for full credit. Please attach the graph to this sheet. 2. Then, graph a double-reciprocal Lineweaver-Burk plot (1/ N0 vs.1/ [S]) to help you determine VmowandK4. a. Attach the graph. Properly label the axes (with units!) and title it "Lineweaver-Burk Plot" for full credit. b. What is the slope and y-intercept of the line? Report your answers on the Answer Sheet for \#2b. Hints for finding slope and y-intercept in Excel are on the next page in the "Hints for Excel" section. 3. What is Vman ? What is Kw ? On the Answer Sheet for \#3, show your work and write your final answers on the line. (Note: use your answer from part 2 to help cakculate this). 4. Now, you are characterizing an inhibitor of the generic entyme, so you repeat your experiment, but incubate it with no inhibitor, 1mM of inhibitor or 2.8mM inhibitor. Your data are in the Excel file on Canvas that looks like helow: a. Graph one double-reciprocal plot (1/Novs. 1/[5]) that contains data from your no inhibitor, 1mM inhibitor, and 2.8mM inhibitor experiments. b. Label the axes properly (with units!) and title it "Lineweaver-Burk Inhibitor Plot" for full credit. c. Fit a line to each set of data. (See above for how to do that in Excel). Attach the graph. Assignment 2 - Enzyme Kinetics, 10 points Directions: You may use any resource (notes, book, etc) and you may work together. Each person must submit his or her own assignment, however. There is an Excel file on Canvas with the data needed to complete the assignment. Please upload 1 file that includes the answers on this answer sheet (either printed out or neatly written on a piece of paper), as well as 3 graphs as described in number 5 below. (The front page of this assignment is your answer sheet that you submit to me; pages 2-3 are directions). Answer Sheet: 2. You graphed a double-reciprocal Lineweaver-Burk plot (1/ V0 vs.1/ [S]) to help you determine Vmax and KM. b. What is the slope and y-intercept of the line? Report your answers on the lines below. Slope: s Y-intercept: s/mM 3. What is Vmax ? What is Km ? Show your work below and write your final answers on the line. (Note: use your answer from part 2 to help calculate this). Vmax: mM/s KM: mM 4. d. What type of inhibitor is this? e. What happens to the KM in this experiment? Circle one: increases decreases stays the same f. What happens to the Vmax in this experiment? Circle one: increases decreases stays the same 5. You may turn in just this answer sheet and the graphs. This is the summary of what graphs should be included/attached to this assignment: a. Michaelis-Menton plot (from \#1) b. Lineweaver-Burk plot (from H2 ) c. Lineweaver-Burk Inhibitor Plot (from \#4) Question 1 data: \begin{tabular}{r|r|} {[S]mM} & V0(mM/s) \\ \hline 0.2 & 1.75381 \\ \hline 0.5 & 3.24371 \\ 1 & 4.52511 \\ 2 & 5.63890 \\ \hline 5 & 6.61596 \\ \hline 10 & 7.02150 \\ \hline \end{tabular} Assignment 2 Directions: Please follow these directions and write your final answers on the Answer Sheet on Page 1. 1. Let's get some practice determining Vma and Ku for a generic encyme. The data table for initial rates for the reaction for various substrate concentrations is on Canvas and will look like the table below. Please open it. a. First, using Excel (preferred) or by graphing by hand, graph the initial rate V0 vs. [S] ( V0 goes on the y-axis and [S] goes on the x-axis). This is a Michaelis-Menton plot. Label the axes with proper units and title it "Michaelis-Menton Plot" for full credit. Please attach the graph to this sheet. 2. Then, graph a double-reciprocal Lineweaver-Burk plot (1/ N0 vs.1/ [S]) to help you determine VmowandK4. a. Attach the graph. Properly label the axes (with units!) and title it "Lineweaver-Burk Plot" for full credit. b. What is the slope and y-intercept of the line? Report your answers on the Answer Sheet for \#2b. Hints for finding slope and y-intercept in Excel are on the next page in the "Hints for Excel" section. 3. What is Vman ? What is Kw ? On the Answer Sheet for \#3, show your work and write your final answers on the line. (Note: use your answer from part 2 to help cakculate this). 4. Now, you are characterizing an inhibitor of the generic entyme, so you repeat your experiment, but incubate it with no inhibitor, 1mM of inhibitor or 2.8mM inhibitor. Your data are in the Excel file on Canvas that looks like helow: a. Graph one double-reciprocal plot (1/Novs. 1/[5]) that contains data from your no inhibitor, 1mM inhibitor, and 2.8mM inhibitor experiments. b. Label the axes properly (with units!) and title it "Lineweaver-Burk Inhibitor Plot" for full credit. c. Fit a line to each set of data. (See above for how to do that in Excel). Attach the graph. Assignment 2 - Enzyme Kinetics, 10 points Directions: You may use any resource (notes, book, etc) and you may work together. Each person must submit his or her own assignment, however. There is an Excel file on Canvas with the data needed to complete the assignment. Please upload 1 file that includes the answers on this answer sheet (either printed out or neatly written on a piece of paper), as well as 3 graphs as described in number 5 below. (The front page of this assignment is your answer sheet that you submit to me; pages 2-3 are directions). Answer Sheet: 2. You graphed a double-reciprocal Lineweaver-Burk plot (1/ V0 vs.1/ [S]) to help you determine Vmax and KM. b. What is the slope and y-intercept of the line? Report your answers on the lines below. Slope: s Y-intercept: s/mM 3. What is Vmax ? What is Km ? Show your work below and write your final answers on the line. (Note: use your answer from part 2 to help calculate this). Vmax: mM/s KM: mM 4. d. What type of inhibitor is this? e. What happens to the KM in this experiment? Circle one: increases decreases stays the same f. What happens to the Vmax in this experiment? Circle one: increases decreases stays the same 5. You may turn in just this answer sheet and the graphs. This is the summary of what graphs should be included/attached to this assignment: a. Michaelis-Menton plot (from \#1) b. Lineweaver-Burk plot (from H2 ) c. Lineweaver-Burk Inhibitor Plot (from \#4)