• You and your lab partner measured the zones of inhibition for the different antibiotics. After measuring the relative size of the zones, one of your clan insisted that the most effective antibiotic against each species was simply the one with the largest zone of inhibition. Your teacher overhead this comment and told your clan that it wasnt quite correct. Explain why you cannot simply compare the size of the zones to determine the most effective drug.
• Diagram the dilution series and spread plating setup you will use to plate the hamburger suspension. Indicate the final dilution for each plate on your diagram.
• Diagram the setup for the water quality testing. Indicate the volumes of water you will inoculate and the initial volume of LSL media in your 9 tubes.
• Does the water testing protocol described in the lab manual involve serial dilution? Justify your answer.
• What products of fermentation will you screen for after incubating the MRVP broth? Describe both the MR and VP test development procedures.
• Diagram the amino acid tryptophan and indicate the bond that is cleaved to produce the indole molecule. How will we test whether indole was produced or not?
• Both the catalase and oxidase tests for enzymes involve in oxygen metabolism. Describe the specific function of each enzyme inside the cell.