You are a virologist looking into the pathology of potential pandemic inducing viruses, whose reservoir are found in wildlife. You investigated the heat shock capacity of different strains of viruses by checking their calcium-binding protein activity, because this is directly related to their capability to survive a fever as immune response of humans. Your measurements of total concentration in of Calcium ions [Ca2+] categorized per temperature are:

20C: 3.2 2.2 3.8 2.8 2.7 3.0

25C: 3.4 2.7 3.2 4.0 2.8 2.9

30C: 3.5 3.7 4.1 3.6 4.7 3.8

35C: 4.5 4.3 4.9 5.1 3.9 4.4

40C: 3.4 3.1 3.6 2.7 3.5 3.2

*NOTE: values are random replicates and are NOT related per column

Investigate the way in which the protein activity changes during different temperature set-ups. Carry out the appropriate inferential test (& post hoc, if needed) and provide the results clearly in a statistical report.

1. "biological question" relating to potential differences/relationship in the data.

2. "statistical null hypothesis" that will best address the question.

3. draft a "descriptive statistics" section, describing quantitative measures of central tendency & dispersion (mean + SE, etc.) and data distribution (G1, G2 values and their description, etc.).

5. draft an "inferential statistics" section, showing the values for

one paragraph on testing assumptions (normal distributions, equal variances, sphericity, etc.) based on the required test for each problem, and

one paragraph providing the answers for the inferential test, and posthoc (if needed)

6. describe a "concluding statement"