You are an analyst in a hospital laboratory. You receive a urine sample from patients. A crude sample from each is tested for pH and dyed with ponceau and indicates the following approximate concentrations:

Patient 1: At least 500 μl/mL Patient 2: At least 5 mg/mL Patient 3: At least 2 mg/mL

Describe the assay you perform with your spectrophotometer that is calibrated for 1 μl/mL to 1 mg/mL protein detection to confirm the concentration of proteins in each patient sample.