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You are designing an experiment to determine whether you can keep cells alive in a 3-D collagen matrix that you fabricated in a Boyden chamber.

You are designing an experiment to determine whether you can keep cells alive in a 3-D collagen matrix that you fabricated in a Boyden chamber. You make a collagen matrix that is 50 m thick and seed it with fibroblasts. The rate of oxygen consumption by cells per unit volume of tissue is e = 310-5 g/cm-3s-1. The diffusivity of oxygen through the tissue is D = 210-5 cm2s-1. At steady state, you determine that the oxygen concentration on one side of the matrix is c0 = 1.810-5 g cm-3, and the concentration on the other side of the matrix is c2 = 0.6 10-5 g cm-3.

Determine the x-position within the matrix where oxygen concentration reaches a minimum.

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