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You are operating the fed - batch reactor for In Class 8 to create a starter culture to manufacture Swiss cheese using Lactobacillus casi. Your

You are operating the fed-batch reactor for In Class 8 to create a starter culture to manufacture Swiss cheese using
Lactobacillus casi. Your client is becoming impatient; they want more cheese, now! You decide to shift to exponential
feed.
Using the same growth parameters as In Class 8: YxS=0.23gx(gS)-1,KS=0.15gL,max=0.7h-1,kd=0.03h-1 :
a. If the initial culture volume was 1000L**, what is the volume after 2.3 hours, in L, if you choose an exponent (power)
equal to the maximum growth rate? Assume that you start from the original feed flow of 4000Lh and are under
isothermal conditions.
b. Under the same conditions as above, what is the specific growth rate after 1.2 hours?
c. What is the cell doubling time after 1.9 hours in hours?
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