You are performing an in vitro experiment in which you will expose a material you are considering for a medical device to synovial fluid, which contains the proteins albumin, transferrin, and IgM at concentrations of 5, 0.5, and 0.05 mg/ml, respectively. Each of these components has a particular affinity for your material, with IgM being the highest and albumin being the lowest.

How would you measure how the concentrations of albumin, transferrin, and IgM on the surface of your biomaterial change over time? For full credit, you must name the method and explain the steps involved.