You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a pathogenic bacteria with the aid of the polymerase chain reaction (PCR). Below is the DNA sequence of the gene (Gene X) that you target for amplification. a) Indicate which set of primers below would you use for the above PCR reaction. Set 1: 5'TACACITATACTTTC 3' and 3' GTAAACCGCGCATTAG 5' Set 2: S' CTCGAGGTGAATAT 3' and 3' CCGCGCATTAGCTAT 5' Set 3: 5' GAGTTACACTTATAC 3' and 3 TGGCGAGTAATCGATA 5 b) While amplifying a DNA sequence using PCR, what are the reaction components that you would need? Why do you need those components for? Please explain. c) Explain why the PCR reaction requires a specific temperature at each step: such as first step at 950C, second step at 550C and third step at 700C for Step 2 You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a pathogenic bacteria with the aid of the polymerase chain reaction (PCR). Below is the DNA sequence of the gene (Gene X) that you target for amplification. a) Indicate which set of primers below would you use for the above PCR reaction. Set 1: 5'TACACITATACTTTC 3' and 3' GTAAACCGCGCATTAG 5' Set 2: S' CTCGAGGTGAATAT 3' and 3' CCGCGCATTAGCTAT 5' Set 3: 5' GAGTTACACTTATAC 3' and 3 TGGCGAGTAATCGATA 5 b) While amplifying a DNA sequence using PCR, what are the reaction components that you would need? Why do you need those components for? Please explain. c) Explain why the PCR reaction requires a specific temperature at each step: such as first step at 950C, second step at 550C and third step at 700C for Step 2