Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a

image text in transcribed

You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a pathogenic bacteria with the aid of the polymerase chain reaction (PCR). Below is the DNA sequence of the gene (Gene X) that you target for amplification. a) Indicate which set of primers below would you use for the above PCR reaction. Set 1: 5'TACACITATACTTTC 3' and 3' GTAAACCGCGCATTAG 5' Set 2: S' CTCGAGGTGAATAT 3' and 3' CCGCGCATTAGCTAT 5' Set 3: 5' GAGTTACACTTATAC 3' and 3 TGGCGAGTAATCGATA 5 b) While amplifying a DNA sequence using PCR, what are the reaction components that you would need? Why do you need those components for? Please explain. c) Explain why the PCR reaction requires a specific temperature at each step: such as first step at 950C, second step at 550C and third step at 700C for Step 2 You have collected a sample from a fresh water stream and want to check the presence of a specific virulence gene that belong to a pathogenic bacteria with the aid of the polymerase chain reaction (PCR). Below is the DNA sequence of the gene (Gene X) that you target for amplification. a) Indicate which set of primers below would you use for the above PCR reaction. Set 1: 5'TACACITATACTTTC 3' and 3' GTAAACCGCGCATTAG 5' Set 2: S' CTCGAGGTGAATAT 3' and 3' CCGCGCATTAGCTAT 5' Set 3: 5' GAGTTACACTTATAC 3' and 3 TGGCGAGTAATCGATA 5 b) While amplifying a DNA sequence using PCR, what are the reaction components that you would need? Why do you need those components for? Please explain. c) Explain why the PCR reaction requires a specific temperature at each step: such as first step at 950C, second step at 550C and third step at 700C for Step 2

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Financial & Managerial Accounting For Undergraduates

Authors: Jason Wallace, James Nelson, Karen Christensen, Theodore Hobson, Scott L. Matthews

2nd Edition

161853310X, 9781618533104

More Books

Students also viewed these Accounting questions

Question

Will you be able to pay your bills?

Answered: 1 week ago