Question
You have discovered a coat color gene (W gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW)
You have discovered a coat color gene (W gene) in rabbits that has two alleles, W and w. Rabbits that are homozygous for W (WW) are red. Rabbits that are homozygous for w (ww) are white. Heterozygous rabbits (Ww) are pink. You have also identified a DNA sequence of about 100 base pairs (probe A) that identifies a 10kb EcoRI fragment in a true-breeding line of rabbits that are red. The same probe identifies a 5kb EcoRI fragment in a true-breeding line of rabbits that are white. When you breed the red rabbits with the white rabbits, you get pink progeny, and the DNA \"probe A described above identifies BOTH a 10kb EcoRI fragment AND a 5kb EcoRI fragment in genomic DNA from the pink rabbits. You can conclude that probe
A is detecting a polymorphic region of the genomic DNA that has two alternate alleles: one we will call A5 as a designation for the RFLP allele you originally found in white rabbits and the other we will call A10 as a designation for the RFLP allele you originally found in the red rabbits. You have identified a second DNA sequence of about 130 base pairs (probe B\") that identifies a 7kb EcoRI fragment in the true-breeding red rabbits and an 11kb EcoRI fragment in the true-breeding white rabbits. We'll call the RFLP allele from the red rabbits B? and the RFLP allele from the white rabbits B11. Genomic DNA from pink rabbits shows both the Band the B11 EcoRI fragments (detected with probe
B). Shown below is a Southern blot in which genomic DNA from WW rabbits (red), Ww rabbits (pink), and ww rabbits (white) has been digested with EcoRI and hybridized to \"probe A\". The genotype and phenotype for the W gene is indicated beneath each lane. The DNA fragment sizes are shown at the left of the blot. The bands in each lane are the DNA fragments detected with probe A. PROBE A kb Gel origin 10 5 WW Ww ww Red Pink white a. Show the fragments you should detect with probe B in the blot drawn below. You should detect one or two bands in each lane of the gel. Indicate their size(s) to the left of the gel (under kb). Write the coat color phenotype below each of the genotypes shown. PROBE B kb Gel origin ww ww ww
Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started