You have grown some cells from a tumour biopsy. You believe that one of the enzymes you are interested in has been mutated. This mutated enzyme is some 60 amino acids shorter in length than its original size. Explain how would could quantify the expression of this mutated enzyme in these cells? Hint: What are some isolation procedures you could use to isolate and purify this enzyme from homogenised cells, and be able to show that it is different to its original unmutated protein. To support your thinking what effect could this mutation have on the enzyme's size and function.