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You just observed your pour plates from the loop dilution method after 48 hrs of growth at 37 degrees C. There is no growth

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You just observed your pour plates from the loop dilution method after 48 hrs of growth at 37 degrees C. There is no growth on plates 2 and 3 (2nd and 3rd dilutions). Please explain at least two errors in technique that could have caused this result.

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