You measure the room temperature hinge angle of a prion based amyloid fiber initially formed at two different temperatures. You image this frame after frame and determine the angle in degrees (see below). Use the equipartition theorem to estimate the torsional stiffness in units of pN*nm/rad. (For help see Hunt and Howard PNAS 90, p11653-11657 (1993) where the torsional stiffness of kinesin was measured.) b Hinge Angle (deg) 200 150 100 50 0 50 37 C Kink 0 4 C Kink 100 150 Frame Ave + 1 S.D. sabo 200 250