You will also need to set up two controls to check that the substrate and enzyme are BOTH required for the reaction to occur. What will they be? Which buffer will you use? Fill in the following table: As part of an experiment examining the effect of pH on enzyme activity you have been asked to prepare 3m reaction solutions with different pH values. Use the following information to fill in the table below to determine how you will prepare your samples for the experiment You will be supplied with: - 0.48M Tris Acetate - Five different 0.48M phosphate buffers at pH4.0,pH6.0,pH7.5,pH9.0 and pH 12.0. - 1.8mM ONPG - 2.4 units/ml of -galactosidase The final concentration of each constituent in the 3ml reaction solutions should be as follows: 0.08M Tris Acetate 0.4 units /ml of -galactosidase 0.3mM ONPG 0.08M phosphate buffers at the appropriate pH (i.e. one of pH4.0,pH6.0,pH7.5,pH9.0,pH12.0 ). You will also need to set up two controls to check that the substrate and enzyme are BOTH required for the reaction to occur. What will they be? Which buffer will you use? Fill in the following table