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Your manager thinks that the lab saves money by making its own stock solutions of protease inhibitors. But, you think you might save money by

Your manager thinks that the lab saves money by making its own stock solutions of protease inhibitors. But, you think you might save money by buying pre-packaged protease inhibitor tablets. Find out which is the least expensive. How much each strategy would cost yearly, for a lab that breaks 100 mL of cells per week

Making it on your own

Protease type

Specific inhibitor

Working concentration

Cost

Serine

PMSF (MW: 174)

Aprotinin

1 mM

10 µg/mL

$50/ 250 mg

$650/ 100 mg

Cys & Ser

Leupeptin

10 µg/mL

$796/ 50 mg

Cysteine

E-64 (MW: 357)

1 mM

$474 / 25 mg

Aspartic Acid

Pepstatin

2 µg/mL

$718/ 100 mg

Metallo-

EDTA (MW 292)

1 mM

$29/ 100 g

Pre made

ThermoFisher sells Pre-made Protease inhibitor with all the above proteases for $749 for 10mL of 100X solution

Based on the yearly calculations from the "pre-made" and "on your own" strategy which one is least expensive and by how much.


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